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Part:BBa_M36294:Design

Designed by: Dominique Dabija   Group: Stanford BIOE44 - S11   (2011-12-06)


Spider Silk Actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
    Illegal NotI site found at 499
    Illegal NotI site found at 1387
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 860
    Illegal BsaI site found at 1748


Design Notes

There were no complications when creating the full sequence. However, some parts, such as the hexamer, took special considerations which are detailed on their basic part pages.


Source

This sequence was used with E. Coli cells.

References